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To test this prediction, we reintroduced chromosomal Lon expression underneath the control of a constitutive promoter within our range strain, and repeated deep mutational scanning in biological triplicate (Supplementary file 2). We consult with the two regimes as +Lon and –Lon selection. The quality of +Lon assortment was similar to that of –Lon collection (Figure 2—determine nutritional supplement 1, Figure 2—determine complement 2). in step with our hypothesis, the distribution of range coefficients shifted in direction of additional adverse values inside the +Lon selection, depleting optimistic collection coefficients and enriching for negative or null coefficients (Figure 2A).
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